Table 2 Effect of glycerol supplementation and L. reuteri PTA5_F13 on the metabolic activity of chicken cecal microbiota during in vitro F2
Reactors | Mean metabolite concentration (mM) ± SD | Ratios of Total SCFAs (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Acetate | Butyrate | Propionate | Succinate | Valerate | 1,3 – PDO | Total BCFAs | Total metabolites | Acetate | Butyrate | Propionate | |
CR | |||||||||||
 Pre-treatment | 91.2 ± 0.9 | 28.8 ± 0.1 | 23.1 ± 1.0 | 7.1 ± 0.8 | 8.3 ± 0.1 | 0.0 ± 0.0 | 14.8 ± 0.2 | 174.1 ± 1.9 | 63.7 ± 0.7 | 20.1 ± 0.1 | 16.2 ± 0.7 |
 Treatment | 95.9 ± 1.0 | 27.2 ± 0.6 | 21.9 ± 0.3 | 6.8 ± 0.2 | 7.1 ± 0.1 | 0.0 ± 0.0 | 14.9 ± 0.2 | 174.0 ± 1.3 | 66.1 ± 0.2 | 15.1 ± 0.2 | 18.8 ± 0.4 |
 Treatment – Pre treatment |  + 4.7 | - 1.6 | - 2.1 | - 0.3 | - 1.2 | - |  + 0.1 | - 0.1 |  + 2.4 | - 5.0 |  + 2.6 |
 P value | 0.0036 | 0.0118 | 0.1220 | 0.6486 | 0.0002 |  | 0.7179 | 0.9254 | 0.0059 | 0.0041 | 0.0606 |
100G | |||||||||||
 Pre-treatment | 96.2 ± 2.6 | 39.8 ± 0.9 | 14.0 ± 0.7 | 4.2 ± 0.4 | 5.7 ± 0.9 | 0.0 ± 0.0 | 13.7 ± 0.2 | 174.0 ± 1.9 | 64.1 ± 0.8 | 26.5 ± 0.5 | 9.3 ± 0.6 |
 Treatment | 61.4 ± 1.7 | 63.3 ± 0.7 | 14.7 ± 1.1 | 3.6 ± 0.2 | 4.8 ± 0.3 | 23.0 ± 1.2 | 12.1 ± 0.2 | 183.4 ± 1.8 | 44.0 ± 0.6 | 45.4 ± 0.9 | 10.6 ± 0.7 |
 Treatment – Pre treatment | - 34.8 |  + 23.5 |  + 0.7 | - 0.6 | - 0.9 |  + 23.0 | - 1.6 |  + 9.4 | - 20.1 |  + 18.9 |  + 1.3 |
 P value | 0.000039 | 0.000004 | 0.3918 | 0.0602 | 0.1644 | 0.000006 | 0.0004 | 0.0036 | 0.000004 | 0.000006 | 0.0915 |
Lbr | |||||||||||
 Pre-treatment | 91.4 ± 1.5 | 32.9 ± 0.3 | 19.9 ± 0.5 | 5.9 ± 0.4 | 8.0 ± 0.2 | 0.0 ± 0.0 | 15.4 ± 0.3 | 173.6 ± 2.3 | 63.4 ± 0.3 | 22.8 ± 0.1 | 13.7 ± 0.3 |
 Treatment | 92.4 ± 1.9 | 36.1 ± 1.2 | 17.0 ± 0.3 | 4.0 ± 0.3 | 6.9 ± 0.7 | 0.0 ± 0.0 | 15.0 ± 0.6 | 171.5 ± 2.7 | 63.5 ± 0.7 | 24.7 ± 0.6 | 11.6 ± 0.4 |
 Treatment – Pre treatment |  + 1.0 |  + 3.2 | - 2.9 | - 1.9 | - 1.1 | - | - 0.4 | - 2.1 |  + 0.1 |  + 1.9 | - 2.1 |
 P value | 0.3524 | 0.0121 | 0.0007 | 0.0017 | 0.0582 |  | 0.4322 | 0.7846 | 0.7857 | 0.0065 | 0.0019 |
Lbr-100G | |||||||||||
 Pre-treatment | 89.6 ± 2.1 | 30.3 ± 0.9 | 27.1 ± 0.6 | 4.3 ± 0.5 | 5.4 ± 1.9 | 0.0 ± 0.0 | 14.9 ± 0.3 | 171.7 ± 3.1 | 60.9 ± 0.9 | 20.6 ± 0.6 | 18.5 ± 0.5 |
 Treatment | 74.0 ± 2.4 | 51.6 ± 0.2 | 22.8 ± 0.3 | 7.7 ± 0.6 | 10.4 ± 0.3 | 20.4 ± 0.3 | 13.2 ± 0.3 | 199.7 ± 3.1 | 49.9 ± 0.7 | 34.7 ± 0.6 | 15.4 ± 0.1 |
 Treatment – Pre treatment | - 15.6 |  + 21.3 | - 4.3 |  + 3.4 |  + 5 |  + 20.4 | - 1.7 |  + 28.0 | - 11.0 |  + 14.1 | - 3.1 |
 P value | 0.0011 | 0.000003 | 0.0004 | 0.0017 | 0.0152 |  < 0.000001 | 0.0022 | 0.0003 | 0.000085 | 0.000007 | 0.0004 |