Fig. 2From: Sub-lethal concentration of a colloidal nanosilver formulation (SilversolĀ®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosaSilver modulates biofilm formation, exopolysaccharide synthesis, protein synthesis, and nitrogen metabolism in P. aeruginosa. (A) Silversol could partially inhibit P. aeruginosa biofilm formation, and eradicate the pre-formed biofilm. Crystal violet assay was performed to quantify biofilm formation, and eradication. Cell viability in the biofilm was estimated through MTT assay; (B) Silversol enhances EPS synthesis in P. aeruginosa. Though there are lesser number of cells in the silver-supplemented media, they synthesized EPS in amount equal to their silver-unexposed counterparts. EPS Unit was calculated as Cell Density (OD764): EPS (g/L) ratio; (C)Ā P. aeruginosa grown in presence of Silversol registered higher protein synthesis. Intracellular and extracellular protein concentrations in P. aeruginosa grown in presence of Silversol at sub-MIC level were significantly higher as compared to its silver-non-exposed counterpart. Kanamycin employed as a positive control at its sub-MIC level also generated similar response from bacterial culture. Protein Unit (i.e., Protein concentration: Cell density ratio) was calculated to nullify any effect of cell density on protein production; (D) Silversol-treated P. aeruginosa culture has higher extracellular accumulation of nitrite. Silversol caused nitrite concentration in P. aeruginosa culture supernatant to rise when compared to control. Sodium nitroprusside (SNP) used as a positive control also caused higher nitrite build up in P. aeruginosa culture. Nitrite Unit (i.e., Nitrite concentration: Cell density ratio) was calculated to nullify any effect of cell density on nitrite production. *pā<ā0.05, **pā<ā0.01, ***pā<ā0.001, ns: non-significantBack to article page