Table 1 Bacterial strains and plasmids used in this study

Bacterial strains

E. coli DH5α

E. coli BL21

F-,φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1

F- ompT hsdSB(rB- mB-) gal dcm (DE3)

This study

Plasmids

pHS10842

pCas9 (Addgene,42,876)

pCas9-sgRNA

pET24

pET24-bla_KPC−2

bla_KPC−2-harboring plasmids

Cm^r, tracRNA, gRNA, and cas9 expression plasmid;

Cm^r, pCas9 cloned with sgRNA targeting bla_KPC−2;

Kan^r, expression vector

Kan^r, recombinant vector derivative with bla_KPC−2 gene

Laboratory stock

[15]

This study

Laboratory stock

This study