Fig. 1
From: Elimination of blaKPC−2-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system
(A) Confirmation of cas9 gene presence in E. coli BL21 + pET24-blaKPC−2 by PCR amplification with primer pCas9-F/R. Lane M represents 2000 bp DNA molecular markers. The 1–20 lanes mean E.coli BL21 + pET24-KPC strain transformed with pCas9-sgRNA, lane N is a negative control. (B) Confirmation of blaKPC−2 gene elimination in BL21 + pET24-blaKPC−2 by PCR amplification with primer blaKPC−2 -F/R in the control group and experimental group
(Note:Integration gel images presented in the main figures are cropped for clarity, full un-cropped gel images are presented in Supplementary Fig.)