Table 3 Intra- and Inter-assay in multiplex detection of SARS-CoV-2 (N gene), Influenza A (M1 gene), and Influenza B (NS1 gene)

Copies of plasmid

Gene

Ct value in Intra-Assay

Mean ± SD

CV%

Ct value in Inter-Assay

Mean ± SD

CV%

1

2

3

Day1

Day2

Day3

5 × 10⁵

N

20.75

21.08

21.56

21.13 ± 0.40

1.93

20.27

20.58

21.68

20.84 ± 0.74

3.55

M1

19.89

19.30

19.30

19.49 ± 0.33

1.74

19.59

20.01

19.80

19.80 ± 0.21

1.06

NS1

18.47

18.46

18.41

18.45 ± 0.03

0.17

18.42

19.22

19.04

18.89 ± 0.42

2.22

5 × 10³

N

26.49

25.43

26.63

26.18 ± 0.65

2.51

26.94

27.62

27.33

27.30 ± 0.34

1.25

M1

26.97

26.57

27.92

27.15 ± 0.69

2.55

25.45

26.42

26.28

26.05 ± 0.52

2.00

NS1

25.13

25.13

25.02

25.10 ± 0.06

0.24

26.81

25.93

26.84

26.53 ± 0.51

1.94

5 × 10¹

N

33.68

33.77

33.12

33.52 ± 0.35

1.05

33.77

35.71

34.81

34.76 ± 0.97

2.79

M1

33.52

31.00

33.09

32.54 ± 1.35

4.15

33.54

33.50

32.72

33.25 ± 0.46

1.39

NS1

31.12

32.39

31.58

31.69 ± 0.64

2.03

32.20

33.10

32.70

32.67 ± 0.45

1.38