Table 2 Primers and probes used in our multiplex RT-qPCR
Assay Primer- probe | Target gene | Oligonucleotide Sequence (5’-3’) | Tm (℃)* | Amplicon size (nt) | Concentration (µM) | Ref. |
|---|---|---|---|---|---|---|
S2V FP RP Probe | N | TACAATGTAACACAACCTTTCGGC GACCTATGTTTGTAATCAGTTCCT TAMRA-CGTGGTACAGAACAAACCCAAGGTAATTTTG-BHQ2 | 63.7 60.7 67.8 | 104 | 0.225 0.225 0.8 | This study |
IAV FP RP Probe | M1 | GACCRATCCTGTCACCTCTGAC AGGGCATTYTGGACAAAKCGTCTA FAM-TGCAGTCCTCGCTCACTGGGCACG-BHQ1 | 64 66 73 | 109 | 0.15 0.15 0.15 | [31] |
IBV FP RP Probe | NS1 | TCCTCAAYTCACTCTTCGAGCG CGGTGCTCTTGACCAAATTGG JOE-CCAATTCGAGCAGCTGAAACTGCGGTG-BHQ1 | 64 63.8 70.7 | 103 | 0.15 0.15 0.15 | [32] |
IC FP RP Probe | RNase P | AGATTTGGACCTGCGAGCG GAGCGGCTGTCTCCACAAGT ROX-TTCTGACCTGAAGGCTCTGCGCG-BHQ2 | 64.4 66.2 70 | 65 | 0.225 0.225 0.5 | [11] |