Fig. 1From: Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for simultaneous detection of SARS-CoV-2 and Influenza A/B virusesA simple workflow for designing and developing of the multiplex assay. For these purposes, (1) in silico approaches, such as gene target selection, multiple alignments, sequences processing and primer-probe sets design, and (2) experimental methods, such as real time PCR optimization, and analytical and clinical evaluation of the assays are crucialBack to article page