Towards unlocking the biocontrol potential of Pichia kudriavzevii for plant fungal diseases: in vitro and in vivo assessments with candidate secreted protein prediction

Table 1 Quantifying the impact of Pk_EgyACGEB_O1 and Pk_EgyACGEB_O2 inoculation on Botrytis cinerea that causes gray mold on the golden delicious apple cultivar

^aWounded golden delicious apple fruits were inoculated with 25 µl 5 × 10⁷ CFUs/ ml Pichia cells and challenged 60 min later with 25 μl 1 × 10⁵ conidia/ml of B. cinerea
^bWounded golden delicious apple fruits were inoculated with 50 µl 5 × 10⁷ CFUs/ ml Pichia cells and challenged 60 min later with 25 μl 1 × 10⁵ conidia/ml of B. cinerea
^cWounds of golden delicious apple fruits inoculated with 25 µl 5 × 10⁷ CFUs/ ml of commercial yeast cells and used as positive control
^dWounds of golden delicious apples treated with 25 µl of sterile distilled water and 60 min later inoculated with 25 µl 1 × 10⁵ conidia/ml of B. cinerea and used as negative control
^eThe average calculated from three fruit replicates
^fAssessment of the infected Area (mm2) after 21 days of treatment

ISSN: 1471-2180