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Fig. 2 | BMC Microbiology

Fig. 2

From: Towards unlocking the biocontrol potential of Pichia kudriavzevii for plant fungal diseases: in vitro and in vivo assessments with candidate secreted protein prediction

Fig. 2

Molecular identification of EgyACGEB_O1 and EgyACGEB_O2 isolates. A PCR amplification of the ITS region of the genomic DNA of EgyACGEB_O1 and EgyACGEB_O2 isolates using the ITS1 and ITS4 primers covered partial part of 18S and 28S as well as 5.8S rRNA genes. B Agarose gel electrophoresis of PCR products showing the amplification of a ~ 600 bp fragment from both EgyACGEB_O1 and EgyACGEB_O2 isolates, original gel (Fig. S1). C Maximum likelihood phylogenetic tree generated using the ITS region sequences of Pk_EgyACGEB_O1, Pk_EgyACGEB_O2, and other Pichia species and related genera. The tree is divided into two clades (I and II) showing the evolutionary relationship between the P. kudriavzevii strains and other related species and genera. Evolutionary analyses were conducted in MEGAX

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BMC Microbiology

ISSN: 1471-2180

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