Fig. 1
From: Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus
Aa Visualization of the checkerboard format: Green boxes represent growth and white boxes inhibition. Orange box shows Fractional inhibition index, pink boxes demonstrate positions of minimal inhibitory concentrations of Fe16 and AMP. Blue box is no-treated S. aureus (Control). Ab image presents antibacterial and synergy activity with MIC and FIC results from checkerboard assay. The values are presented as the average from three independent experiments. Ac Isobologram showing the synergy effect of Fe16 and AMP with FIC index ≤ 0.5 and table demonstrating the the FIC index category scale. B External morphological changes of S. aureus after exposure to Fe16+AMP assessed by SEM. Figure Ba shows untreated S. aureus as a control, Bb demonstrates S. aureus treated with Fe16 and Bc AMP alone at 0.125 ug/ml. Bd is S. aureus treated with Fe16+AMP at 0.25 ug/ml. Red arrows indicate S. aureus morphological changes. C Cytotoxic effects of Fe16, AMP and Fe16+AMP at the different concentrations on the HaCaT keratinocyte cell line