Reaction system | 2 × mix 20 µL, Primer27F 1.5 µL, Primer1492R 1.5 µL, ddH2O 15 µL, Template gDNA 2 µL | |||
Reaction process | Predenaturation | Transsexual | Refolding | Extend |
Temperature Reflex | 95 ℃ | 95 ℃ | 52 ℃ | 72 ℃ |
Reaction time | 3Â min | 30Â s | 30Â s | 7Â min |