Fig. 6

Isolation and identification of exosomes. A. Representative electron microscopy images are shown. Scale bar = 100 nm. ←, exosomes. (A-A) Electron microscopy results of the pcDNA 3.1 cell pellet. (A-B): Electron microscopy results of the Ats-1 cell pellet. (A-C) Electron microscopy results of the siRNA SDCBP + Ats-1 cell pellet. B. Representative nanoparticle tracking analysis (NTA) traces of exosomes derived, normalized to the cell number. (B-A) NTA traces of exosomes derived from the pcDNA 3.1 cells. (B-B) NTA traces of exosomes derived from the Ats-1 cells. (B-C) NTA traces of exosomes derived from the siRNA SDCBP + Ats-1 cells. C. NTA quantification of three independent experiments. D. Western blot analysis of microvesicles purified from equal numbers of the pcDNA 3.1, Ats-1, and siRNA SDCBP + Ats-1 cells. The blots were cut prior to hybridisation with antibodies during blotting. The results in the figure were obtained from three independent replicate experiments. E. Quantification of the exosomal protein levels in the microvesicles obtained from the pcDNA 3.1, Ats-1, and siRNA SDCBP + Ats-1 cells in the three independent experiments. All data are shown as mean ± SD from three independent tests. ns, no significant difference, **p < 0.01, ***p < 0.001, ****p < 0.0001