Fig. 4

qPCR of the expression levels of SDCBP and its related proteins induced by Ats-1. siRNA SDCBP was transferred into the HEK293T cells, and 24 h later, pcDNA 3.1 or Ats-1 was transferred into the HEK293T cells. After 48 h, total cellular RNA was extracted and reverse transcribed into cDNA for qPCR analysis. A. mRNA expression level of SDCBP after siRNA SDCBP 2/5/7 transfer. B. mRNA expression level of SDCBP before and after siRNA SDCBP /Ats-1 transfer. C. mRNA expression level of SDC1 before and after siRNA SDCBP/Ats-1 transfer. D. mRNA expression level of SDC2 before and after siRNA SDCBP/Ats-1 transfer. E. mRNA expression level of SDC4 before and after siRNA SDCBP/Ats-1 transfer. F. mRNA expression level of IL5 before and after siRNA SDCBP/Ats-1 transfer. G. mRNA expression level of ANXA2 before and after siRNA SDCBP/Ats-1 transfer. H. mRNA expression level of EFNB1 before and after siRNA SDCBP/Ats-1 transfer. I. mRNA expression level of NFASC before and after siRNA SDCBP/Ats-1 transfer. All data are shown as mean ± SD from three independent tests. ns, no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001