Fig. 1

Ats-1 structure prediction and expression. A. Schematic diagram of the Ats-1 protein structure, where light yellow indicates the mitochondrial localization signal, and green indicates the antigenic determinant. Ats-1 had a total of 10 antigenic determinants. The numbers indicate the amino acid sites. The specific amino acid sites of each protein structure are provided in Supplementary Fig. 1. B. Expression of Ats-1 in HEK293T cells. After pcDNA3.1-Ats-1 was transferred to the HEK293T cells, samples were collected for Western blot analysis at 24 and 48 h. The blots were cut prior to hybridisation with antibodies during blotting. C. Western blot results were semi-quantitatively analyzed using ImageJ software. All data are shown as mean ± SEM from three independent tests. *p < 0.05