Highly sensitive and rapid determination of Mycobacterium leprae based on real-time multiple cross displacement amplification

Table 3 The details of primers for the RLEP gene

Gene^a	Primers name	Sequences and modifications^b	Length^c
RLEP	RLEP-F1	5’-GCGGGGTTTTTGTTGGTG-3’	18 nt
	RLEP-F2	5’-CACACTAAAACGCCCCTACC-3’	20 nt
	RLEP-CP1	5’-CCGCGCTAATCGTGTCCACTG-GGCTGACTGCCTGCTTTC-3’	39 mer
	RLEP-CP2	5’-ATGTTGATGATGCCAGGGGCT-ATCCTCACACTCACGCCT-3’	39 mer
	RLEP-C1	5’-CCGCGCTAATCGTGTCCACTG-3’	21 nt
	RLEP-C2	5’-ATGTTGATGATGCCAGGGGCT-3’	21 nt
	RLEP-D1*	5’-FAM-TGCAATG-AAAGCACACGAAGCCT(BHQ1)CATC-3’	27 nt
	RLEP-D2	5’-GCACCTGGGCTGTGCTGAA-3’	19 nt
	RLEP-R1	5’-GCACGTAAGCATGTCGGT-3’	18 nt
	RLEP-R2	5’-AGACCAAGCAGCATCCAC-3’	18 nt

^aRLEP, the repetitive element gene of ML; ^bFAM, 6-carboxy-fluorescein; BHQ1, black hole quencher 1; ^cmer, monomeric unit; nt, nucleotide

ISSN: 1471-2180