Fig. 3
Analytical sensitivity of the E-RT-MCDA assay targeting the RLEP gene. A The serial fivefold dilutions (a 1.9 × 107 fg/µl, b 3.8 × 106 fg/µl, c 7.6 × 105 fg/µl, d 1.52 × 105 fg/µl, e 3.04 × 104 fg/µl, f 6.08 × 103 fg/µl, g 1.22 × 103 fg/µl, h 2.43 × 102 fg/µl, i 4.86 × 101 fg/µl, j 9.73 fg/µl, and k 1.95 fg/ul) of ML DNA shall be tested according to the standard E-RT-MCDA. (l) Double-distilled water (DW) was used as a blank control (BC). B The CT was plotted against the DNA concentration of the positive reactions. The ordinate is the number of PCR cycles at the threshold and the abscissa is the number of ML DNA(log10)