Fig. 2
Optimal reaction temperature for the ML MCDA assay. ML genomic DNA was subjected to the standard MCDA reaction at the level of 1.52 × 105 fg per mixture, and monitored by real-time turbidimetry. Different reaction temperatures were then used for the tests, ranging from 63 to 70 °C, with intervals of 1 °C. The threshold value of turbidity ≥ 0.1 was considered positive amplification. (A1-A8) Real-time turbidity kinetic graphs. (B1-B8) The accumulation turbidity kinetic graphs. (CH1) The ML genomic DNA at 1.52 × 105 fg per mixture. (CH2) Negative control (NC) containing M. tuberculosis genomic DNA. (CH3) Double-distilled water (DW) used as the blank control (BC)