Fig. 6
The profile of antimicrobial activity on Ligilactobacillus salivarius. A The inhibitory action of Ligilactobacillus salivarius on pathogen and the metabolic profiles. The biosynthesis pathway was annotated depending on KEGG. The graph of metabolic pathway was found through enriched of KEGG metabolic pathway after collecting the metabolites through UPLC-MS detection. Clavulanic acid produced by L. salivarius and their pathway were manifested comprehensively. The pathway was originated from the metabolism of arginine through the cascade catalyzed reactions, the processed substances were L-N2-(2-Carboxye thyl)-arginie, Deoxyguanidion-proclavaminic acid, Guanidino- proclavaminic acid, Proclavaminic acid, Dihydroclavaminic acid, Clavaminic acid, Clavulanate-9-aldehyde in sequenced. Clavalanic acid was produced in the end. B The inhibitory action of Ligilactobacillus salivarius on pathogen. The inhibitory action of Lactobacillus salivarius on pathogen. The classic cylinder-plate method was employed to evaluate the antimicrobial activity. Three strains of common pathogenic bacteria: Escherichia coli, Salmonella, Staphylococcus aureus were used as the test object. The inhibitory zones were measured to evaluate the antimicrobial capacity