Fig. 4

Alpha diversity indices of bacterial communities with and without treatment with AITC. (a) Raw CCS data: the number of CC sequences in each sample; Clean CCS data: the number of sequences after primer removal and length filtering (< 0.005%). (b) Number of species types at each level (phylum, order, and genus) of the soil bacterial community. (c and d) Alpha diversity analysis including the Chao1, ACE, Simpson, and Shannon indices. n = 3 for CC/AITC treatment group. All data are presented as mean ± standard deviation (SD) and were compared using two-way ANOVA.