Table 1 Strains of E. coli and plasmids

BL21 (DE3)

F– ompT gal dcm lon hsdS_B(r_B^–m_B^–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB⁺]_K−12(λ^S) pLysS[T7p20 ori_p15A](Cam^R)

[38]

BTH101

F- cya-99, araD139, galE15, galK16, rpsL1, hsdR2, mcrA1, mcrB1 (Str^R)

[19]

C43 (DE3)

Derivative of BL21 (DE3), F − ompT, gal, dam, lon, hsdS_B(r_B^-m_B^-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

[39]

Plasmid

Genotype

Reference

pMW3043

pET28a, but with His₆-CyaC

This work

pMW3065

pASK-IBA3, but with CycR-Strep

This work

pMW3084

pET28a, but with His₆-Clr

This work

pMW3085

pASK-IBA3plus, but with Clr-Strep

This work

pKT25

pSU40, but for C-terminal T25 fusion, Km^R

[19]

pKNT25

pSU40, but for N-terminal T25 fusion, Km^R

[19]

pUT18

pUC19, but for N-terminal T18 fusion, Ap^R

[19]

pUT18C

pUC19, but for C-terminal T18 fusion, Ap^R

[19]

His₆-pKT25

pKT25 derivative, but with His₆-Tag

[40]

His6-pKNT25

pKNT derivative, but with His₆-Tag

[20]

His6-pUT18

pUT18 derivative, but wih His₆-Tag

[20]

His₆-pUT18C

pUT18C deriative, but with His₆-Tag

[40]

pKNT25-zip

pKNT25, but T25-zip, Km^R

[19]

pUT18-zip

pUT18, but T18-zip, Ap^R

[19]

pMW689

His₆-pUT18 derivative with CyaC^# [CyaC(K410/T484A)]

This work

pMW692

His₆-pKNT25 derivative with CyaC^# [CyaC(K410/T484A)]

This work

pMW690

His₆-pUT18 derivative with CyaC^## [CyaC(R495A/N491A)]

This work

pMW691

His₆-pKNT25 derivative with CyaC^## [CyaC(R495A/N491A)]

This work

pMW1424

pUT18C, butT18-NreC (S. carnosus), Ap^R

[41]

pMW1439

pUT18, but NreC-T18 (S. carnosus), Ap^R

[41]

pMW1777

pKNT25, but NreC-T25 (S. carnosus), Km^R

[41]

pMW1780

pKT25, but T25-NreC (S. carnosus), Km^R

[41]

pMW2958

His₆-pKNT25, but CycR-T25, Km^R

This work

pMW2959

His₆-pKT25, but T25-CycR, Km^R

This work

pMW2961

His₆-pUT18C, but T18-CycR Amp^R

This work

pMW3087

pUT18, but Clr-T18, Amp^R

This work

pMW3088

pUT18C, but T18-Clr, Amp^R

This work

pMW3090

pKNT25, but Clr-T25, Km^R

This work