Fig. 5
Binding of His6-CyaC to CycR-Strep. The graphs show SPR experiments, where CycR-Strep was captured onto a CM5 sensor chip previously immobilized with StrepTactin. First, approximately 200 Response Units of CycR-Strep were captured on the chip, and then different concentrations of His6-CyaC were injected [10 nM (dark green line), 25 nM (red line), 50 nM (light green line), 100 nM (yellow line), 2 × 250 nM (brown and blue line), 500 nM (turquoise line), 1.000 nM (light blue line), 2.500 nM (pink line), and 5.000 nM (brown line)]. Between each cycle, the chip was regenerated and new CycR-Strep was captured. The experiments were performed in the absence (A) and presence (B) of 500 µM cAMP. The pictures represent characteristic of at least three independent experiments