Fig. 4

Co-sedimentation of CycR-Strep with Clr-His₆ bound to magnetic particles. His₆-Clr (29.3 µg) was incubated in 500 µl binding buffer containing CycR-Strep (28.3 µg), ATP (3.5 mM) and cAMP (0.5 mM) as indicated. After separation of the beads and washing, the proteins associated with the beads were separated by SDS-PAGE and tested by Western-blotting for Clr (anti-His₆ antiserum, upper panel) and CycR (anti-Strep antiserum, lower panels) separately. Experiments were performed in two or more repeats. The positions of His₆-Clr (29.3 kDa) and CycR-Strep (28.3 kDa) in the Westernblot are indicated by the protein marker of 25 kDa. The full length blots are shown in Figure S3