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Fig. 3 | BMC Microbiology

Fig. 3

From: A signaling complex of adenylate cyclase CyaC of Sinorhizobium meliloti with cAMP and the transcriptional regulators Clr and CycR

Fig. 3

Co-sedimentation of CycR-Strep with His6-CyaC bound to magnetic particles. His6-CyaC (64 µg) was incubated in 500 µl binding buffer containing CycR-Strep (28.3 µg), ATP (3.5 mM) and cAMP (0.5 mM) as indicated. After separation of the beads and washing, the proteins associated with the beads were separated by SDS-PAGE and tested by Western-blotting for CyaC (anti-His6 antiserum, upper panel) and CycR (anti-Strep antiserum, lower panel) separately. Experiments were performed in two or more repeats. The positions of His6-CyaC (64.0 kDa) and CycR-Strep (28.3 kDa) in the Westernblot are indicated by protein markers of 25 und 55 kDa. The full length blots are shown in Figure S2

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BMC Microbiology

ISSN: 1471-2180

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