Fig. 2

Co-sedimentation of Clr-Strep with (A) His₆-CyaC and (B) His₆-CyaC^# bound to magnetic beads. His₆-CyaC (64 µg) or His₆-CyaC^# (64 µg) were incubated in 500 µl binding buffer containing Clr-Strep (29.6 µg), ATP (3.5 mM) and cAMP (0.5 mM) as indicated. After separation of the beads and washing, the proteins associated with the beads were separated by SDS-PAGE and tested by Western-blotting for CyaC (anti-His₆ antisera, upper panels) and Clr (anti-Strep antisera, lower panels) separately. All experiments were performed in two or more repeats. The positions of His₆-CyaC (64.0 kDa) and Clr-Strep (29.6 kDa) in the Westernblot are indicated by the protein markers of 25 und 55 kDa. The full length blots are shown in Figure S1