Fig. 1

Interaction of CyaC, Clr and CycR tested by the BACTH system for the pairs CyaC^#/ Clr (A), CyaC^#/CycR (B), Clr / CycR (C), and the non-related gene regulator NreC (D) in E. coli BTH101 Proteins CyaC^# (Cyac(K410A T484A), Clr and CycR were fused C- or N-terminally to the T18 or T25 fragments as indicated, and produced by pairwise co-expression of the corresponding genes from plasmids (Table 1). The Leu zipper (Zip) pair fused to T18 and T25 fragments, respectively, was used as the positive control, the pair Zip-T25 with CyaC^#-T18 as the negative control. 100% activity corresponds to 1,450 Miller-Units in (A) and (C), 2,700 Miller-Units in (B), and 1.630 Miller-Units in (D). Bacteria were grown anaerobically in LB medium supplemented with 20 mM dimethylsulfoxide. β-Galactosidase activities are given in Miller-Units MU [17] as the mean (with standard deviation) from at least two biological and four technical replicates each