Fig. 6
Verification of differential TSS expression. A Relative read scores (RRS) for TSS of annotated genes (ecpR, flhD, fliA) and asTSS at genome positions 4,763,189 (asTSS No. 1) and 2,742,524 (asTSS No. 2) from Cappable-seq libraries. Mean RRS of three biological replicates and the standard deviation are given. Differential expression was statistically verified at FDR < 0.05 and log2 FC >|2| between LB and salt supplemented LB in all instances. Orange, LB in exponential phase; red, LB + salt in exponential phase; light blue, LB in stationary phase; dark blue, LB + salt in stationary phase. B Normalized expression (ΔΔCq) of ecpR, flhD, fliA, and of asTSS No. 1 and asTSS No. 2 according to Fig. 6A. Quantification was performed with RT-qPCR. Expression of the gene cysG (siroheme synthase) was used for normalization. Significant up- or down-regulation in salt-supplemented medium was statistically significant for ecpR, asTSS No. 1, and asTSS No. 2 or flhD and fliA, respectively (one-tailed paired t-test, * p ≤ 0.05; ** p ≤ 0.01). Orange, LB in exponential phase; red, LB + salt in exponential phase; light blue, LB in stationary phase; dark blue, LB + salt in stationary phase