Fig. 3

Microbial features at the genus level of pregnant women with ICP. A Heatmap of the relative abundance of gut microbiome constituents at the genus level of pregnant women in the control, mild ICP and severe ICP groups (clustered by group, H stands for severe ICP group, L for mild ICP group and N for control group): The community composition of differentially abundant genera was clustered according to the abundance distribution of taxa or the degree of similarity between samples. The taxa and samples were sorted separately according to the clustering results. Through clustering, high- and low-abundance taxa were distinguished, and the color gradient reflects the similarity of community composition between samples. B Association of differentially abundant gut microbiome constituents at the genus level for all enrolled pregnant women (clustered by family): A Spearman correlation test was performed to evaluate the relationships among differentially abundant genera among the groups. Red lines show positive associations, and green lines show negative associations. C Association of clinical characteristics and differentially abundant gut microbiome constituents at the genus level for all enrolled pregnant women. D-F Regression analysis of TBA levels and the abundances of Escherichia-Shigella, Ruminococcaceae UCG-014 and (Eubacterium) coprostanoligenes groups