Pre-treatment with Dazomet enhances the biocontrol efficacy of purpureocillium lilacinum to Meloidogyne incognita

Table 3 Nematicidal activity of Dazomet and its effect on the endurance of M. incognita J₂s to the fermentation filtrate of P. lilacinum YES-2-14

Dosage of Dazomet (mg kg^− 1 soil)	J₂ mortality in Dazomet treatment (%)	J₂ mortality in combined treatment (%)^#	P	M (%)^†
0	0.7 ± 0.1 a	7.4 ± 1.4 a	0.0156	7.4
2.5	2.2 ± 0.8 ab	8.5 ± 1.0 a	0.0012	8.8
5	3.1 ± 2.4 b	10.0 ± 0.5 a	0.0082	9.6
10	5.4 ± 1.7 c	24.8 ± 3.1 b	0.0007	11.8
20	52.1 ± 1.5 d	95.0 ± 1.3 c	< 0.0001	55.0

Data are means ± standard deviations of three independent replicates. Values within a column followed by the same letter are not significantly different at P < 0.05 level according to Duncan’s multiple range test.
# Values in this column represent J₂ mortality caused by the combined treatment of Dazomet fumigation followed by the 10-fold diluted fermentation filtrate of P. lilacinum YES-2-14.
P represents statistical difference between J₂ mortality post fumigation and J₂ mortality post fumigation and fungal filtrate using the t test.
^†MT represents the theoretical mortality of J₂s treated with Dazomet and P. lilacinum successively. MT (%) = 1 - (1-mr) × (1- mo), in which mr and mo represent the revised mortality of J₂s in the treatments of Dazomet fumigation and fungal filtrate respectively.

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