Fig. 5From: Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotesGelatinolytic activity of metalloproteinases in promastigote supernatant. (A) Zymography gel of La. (B) Zymography gel of Lb. (C) Comparison of gelatinase activity for La. (D) Comparison of gelatinase activity for Lb. (E) MMP-2 activity of La, with or without DOX. (F) MMP-2 activity of Lb, with or without DOX. (G) MMP-9 activity of La, with or without DOX. (H) MMP-9 activity of Lb, with or without DOX. Optical densitometry comparison between Leishmania species: (I) MMP-2 and (J) MMP-9. LOG: logarithmic growth phase (2 days). STAT: stationary phase of growth (7 days). LSTAT: late stationary phase of growth (9 days). FBS: fetal bovine serum. R: RPMI + FBS. DOX: doxycycline (50 µM). **p < 0.01, *p < 0.05Back to article page