Gamma rays-assisted bacterial synthesis of bimetallic silver-selenium nanoparticles: powerful antimicrobial, antibiofilm, antioxidant, and photocatalytic activities

Table 5 DPPH free radical scavenging activity of Ag NPs, Se NPs, and bimetallic Ag-Se NPs

NPs concentration (µg mL^− 1)	Free radical scavenging activity (%)
NPs concentration (µg mL^− 1)	Ascorbic acid	Ag NPs	Se NPs	Ag-Se NPs
0.00 (C)	0.00^g	0.00^h	0.00^h	0.00^g
25	20.56 ± 2.56^f	15.11 ± 1.36^g	17.21 ± 1.45^g	19.32 ± 2.56^f
50	47.65 ± 3.45^e	27.87 ± 1.41^f	36.76 ± 3.54^f	38.53 ± 5.15^e
100	55.43 ± 4.87^d	38.67 ± 4.52^e	51.81 ± 2.61^e	50.32 ± 3.64^d
200	61.08 ± 6.33^c	48.56 ± 4.87^d	59.59 ± 5.44^d	58.56 ± 1.56^c
400	75.22 ± 8.51^b	62.06 ± 3.56^c	68.54 ± 5.42^c	68.65 ± 4.48^b
800	98.71 ± 6.32^a	75.17 ± 2.66^b	74.32 ± 7.06^b	89.34 ± 3.42^a
1000	100.00 ± 0.00^a	83.65 ± 7.51^a	82.41 ± 6.11^a	91.14 ± 7.85^a
IC₅₀(µg mL^− 1)	71.61	242.54	124.53	98.52

DPPH scavenging assay was used for measuring the antioxidant activities of the synthesized nanoparticles at 517 nm using DPPH solution under the conditions described in Materials and Methods. Calculated mean is for triplicate measurements from three independent experiments ± SD, ^a−hmeans with different superscripts in the same column are considered statistically different (LSD test, P ≤ 0.05)

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