Table 1 Primers for virulence genes used in qRT-PCR
From: Crippling of Klebsiella pneumoniae virulence by metformin, N-acetylcysteine and secnidazole
Gene name | Primer sequence (5′ → 3′) | Reference |
|---|---|---|
khe | F: GATGAAACGACCTGATTGCATTC | [31] |
R: CCGGGCTGTCGGGATAAG | ||
fimH-1 | F: GCC AAC GTC TAC GTT AAC CTG | [32] |
R: ATA TTT CAC GGT GCC TGA AAA | ||
mrkD | F: AAGCTATCGCTGTACTTCCGGCA | [33] |
R: GCGTTGGCGCTCAGATAGG | ||
ureA | F: GACAAGCTGTTGCTGTTTACC | [34] |
R: CGGGTTGTGAACGGTGAC | ||
rmpA | F; ACTGGGCTACCTCTGCTTCA | [35] |
R: CTTGCATGAGCCATCTTTCA | ||
wcaG | F: GGTTGGKTCAGCAATCGTA | [32] |
R: ACTATTCCGCCAACTTTTGC | ||
16Â s rRNA | F: GCAAGTCGAGCGGTAGCACAG | [36] |
R: CAGTGTGGCTGGTCATCCTCTC |