Table 1 Primer pairs used for the detection of Brucella genus and Brucella species
Target pathogen | Primer | Sequences | Amplified Products | References |
|---|---|---|---|---|
Brucella, BCSP31 | B4 (F) | 5ʹ-TGGCTCGGTTGCCAATATCAA-3ʹ | 223 bp | [29] |
B5 (R) | 5ʹ-CGCGCTTGCCTTTCAGGTCTG-3ʹ |  | ||
B. abortus BA | F | 5ʹ-CCATTGAAGTCTGGCGAGC-3ʹ | 156 bp | [30] |
R | 5ʹ-CGATGCGAGAAAACATTGACCG-3ʹ |  | ||
B. melitensis IS711 | F | 5ʹ-TGCCGATCACTTAAGGGCCTTCAT-3ʹ | 731 bp | [31] |
R | 5ʹ-AAA TCGCGTCCTTGCTGGTCTGA-3ʹ |  |