Occurrence of Acinetobacter baumannii genomic resistance islands (AbGRIs) in Acinetobacter baumannii strains belonging to global clone 2 obtained from COVID-19 patients

Table 5 Primer pairs used for mapping of AbGRI2

PCR	Primer	Sequence (5’-3’)	Annealing temperature (° C)	Amplicon length (bp)	Reference
AB57_1175 - tnpR₁	RH1315 RH539	AGGAGATCTTCTTGGCAGTCA CCAGCCCTTCCCGATCTGTTG	60	1051	[10]
bla _TEM− tnpA ₁₀₀₀	RH605 RH759	TTTCGTGTCGCCCTTATTCC GCCAGCTCATTTACCTTGCCGA	60	2650	[10]
tnpR _5393c -aphA1b	RH520 RH880	CATGGCCCAGCGCGATACTTCAG CAACGGGAAACGTCTTGCTC	60	2297	[10]
aphA1b- sul1	RH881 RH751	ATTCGTGATTGCGCCTGAG GCGGAACTTCACGCGATC	60	2712	[10]
tnpA ₂₁ -AB57_1209	RH668 RH1316	CACCAGAACCGCCTGCTCAA CATCTGCCATCCAGTTTGTG	60	1219	[10]
TE32_13140-tnpR1	RH1563 RH539	ATAGATCGGCTTCGGACTCA CCAGCCCTTCCCGATCTGTTG	60	1046	[9]
aphA1b-ABA1_01228	RH881 RH2008	ATTCGTGATTGCGCCTGAG TGATGACTTCCATTAAAGCCTGT	60	1581^a	[9]
*tnpR* _5393c - ABA1_01228	RH520 RH2008	CATGGCCCAGCGCGATACTTCAG TGATGACTTCCATTAAAGCCTGT	60	1500	[9]
bla _TEM	RH605 RH606	TTTCGTGTCGCCCTTATTCC CCGGCTCCAGATTTATCAGC	60	690	[8]
sul1	HS549 HS550	ACTAAGCTTGCCCCTTCCGC CTAGGCATGATCTAACCCTCG	60	1100	[29]
aphA1b	RH880 RH881	CAACGGGAAACGTCTTGCTC ATTCGTGATTGCGCCTGAG	60	454	[30]
aacC1	RH935 RH936	GCAGTCGCCCTAAAACAAAG CCCGTATGCCCAACTTTGTA	60	457	[8]
aadA1	RH522 RH531	GTGGATGGCGGCCTGAAGCCA GGCAGCGACATCCTTCGGCGC	60	516	[8]
aacC1-aadA1	RH935 RH531	GCAGTCGCCCTAAAACAAAG GGCAGCGACATCCTTCGGCGC	60	2708	[8]

a. Predicted size based on AbGRI2-12a. For AbGRI2-12b it is 1,772
PCR in bold is the linkage PCR that were performed for identification of AbGRI1s in this study

ISSN: 1471-2180