Table 5 Target genes, primer sequences, and annealing temperatures used in this study
Target genes | Primer sequence (5´-3´) | PCR product size (bp) | Annealing Tm (˚C) and Time (S) | Reference |
|---|---|---|---|---|
icaA | F-TCTCTTGCAGGAGCAATCAA R-TCAGGCACTAACATCCAGCA | 188 | 58 ËšC for 30s | (34) |
icaB | F-ATGGCTTAAAGCACACGACGC R-TATCGGCATCTGGTGTGACAG | 526 | 50 ËšC for 30s | (24) |
icaC | F-ATCATCGTGACACACTTACTAACG R-CTCTCTTAACATCATTCCGACGCC | 934 | 50 ËšC for 30s | (24) |
icaD | F-ATGGTCAAGCCCAGACAGAG R-CGTGTTTTCAACATTTAATGCAA | 198 | 55 ËšC for 30s | (34) |
aap | F-ATACAACTGGTGCAGATGGTTG R-GTAGCCGTCCAAGTTTTACCAG | 400 | 50 ËšC for 30s | (13) |
atlE | F-CAACTGCTCAACCGAGAACA R-TTTGTAGATGTTGTGCCCCA | 682 | 62 ËšC for 60s | (13) |
bhp | F-ACGGACAATATCGTCTCTCAA R-AACTTCAGCCGTTCCCTT | 1917 | 55 ËšC for 30s | (13) |
embp | F-AGCGGTACAAATGTCAATATC R-AGAAGTGCTCTAGCATCATCC | 455 | 62 ËšC for 60s | (13) |
fbe | F-TAAACACCGACGATAATAACCAAA R-GGTCTAGCCTTATTTTCATATTCA | 496 | 62 ËšC for 60s | (13) |
arcA | F-CTAACACTGAACCCCAATG R-GAGCCAGAAGTACGCGAG | 1946 | 52 °C, 1 min; | (13) |
opp3AB | F-GCAAATCTGTAAATGGTCTGTTC R-GAAGATTGGCAGCACAAAGTG | 1183 | 52 °C, 1 min; | (13) |