Table 1 List of the primers that were used in qPCR amplification process
From: Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa
Name | Forward primer (5’–3’) | Reverse primer (5’–3’) | Amplicon length (bp) | Tm (ºC) |
|---|---|---|---|---|
lasI | CTTTTCCGACTGTACGCT | ATCATCTTCTCCACGCCT | 108 | 57 |
lasR | CTTGGTTGACGGTTTTCT | CTCCACTCCAATTTTCCAC | 54 | 57 |
rhlI | CAAACCCGCTACATCGTC | TGCACAGGTAGGCGAAGA | 109 | 56 |
rhlR | GAGGCTTTTTGCTGTGGT | GGGTGAAGGGAATCGTGT | 105 | 56 |
algD | GGGGCCAACAAGGAATACA | AGCACCAGCACATCGGAA | 101 | 57 |
gyrA | ACACCGAGGCGAACAAGA | TAGTCGGTGGTGAGGAAGT | 73 | 57 |