Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles

Ahmed, Heba A.; Elsohaby, Ibrahim; Elamin, Amina M.; El-Ghafar, Abeer E. Abd; Elsaid, Gamilat A.; Elbarbary, Mervat; Mohsen, Rasha A.; El Feky, Tamer M.; El Bayomi, Rasha M.

doi:10.1186/s12866-023-02948-0

Table 1 Primers, product size and annealing temperatures used for identification, virulence and antimicrobial resistance genes reported in the present study

From: Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles

Gene	Primer sequences (5’-3’)	Product size (bp)	Annealing (°C)	References
hlyA	fw: AACAAGGATAAGCACTGTTCTGGCT	1177	60˚C	[28]
	rev: ACCATATAAGCGGTCATTCCCGTCA	1177	60˚C	[28]
phoA	fw: CGATTCTGGAAATGGCAAAAG	720 bp	60˚C	[25]
phoA	rev: CGTGATCAGCGGTGACTATGAC	720 bp	60˚C	[25]
stx1	fw: ACACTGGATGATCTCAGTGG	614	58˚C	[30]
	rev: CTGAATCCCCCTCCATTATG	614	58˚C
stx2	fw: CCATGACAACGGACAGCAGTT	779	58˚C
stx2	rev: CCTGTCAACTGAGCAGCACTTTG	779	58˚C
astA	fw: CCATCAACACAGTATATCCGA	110	55˚C	[29]
astA	rev: GGTCGCGAGTGACGGCTTTGT	110	55˚C	[29]
stx2f	fw: AGA TTG GGC GTC ATT CAC TGG TTG	428	57˚C	[31]
stx2f	rev: TAC TTT AAT GGC CGC CCT GTC TCC	428	57˚C	[31]
fimH	fw: TGCAGAACGGATAAGCCGTGG	508	50˚C	[32]
fimH	rev: GCAGTCACCTGCCCTCCGGTA	508	50˚C	[32]
eaeA	fw: ATG CTT AGT GCT GGT TTA GG	248	51˚C	[33]
eaeA	rev: GCC TTC ATC ATT TCG CTT TC	248	51˚C	[33]
blaIMP	fw: CATGGTTTGGTGGTTCTTGT	488	53˚C	[39]
blaIMP	rev: ATAATTTGGCGGACTTTGGC	488	53˚C
blaVIM	fw: AGTGGTGAGTATCCGACA	280	53˚C
blaVIM	rev: ATGAAAGTGCGTGGAGAC	280	53˚C
blaNDM	fw: GGCGGAATGGCTCATCACGA	287	55˚C
blaNDM	rev: CGCAACACAGCCTGACTTTC	287	55˚C
blaTEM	fw: ATCAGCAATAAACCAGC	516	54˚C	[40]
blaTEM	rev: CCCCGAAGAACGTTTTC	516	54˚C
blaOXA-1	fw: ATATCTCTACTGTTGCATCTCC	619	54˚C
blaOXA-1	rev: AAACCCTTCAAACCATCC	619	54˚C
blaSHV	fw: AGGATTGACTGCCTTTTTG	392	54˚C
blaSHV	rev: ATTTGCTGATTTCGCTCG	392	54˚C
blaCMY	fw: GACAGCCTCTTTCTCCACA	1143	60˚C	[41]
blaCMY	rev: TGGAACGAAGGCTACGTA	1143	60˚C	[41]
blaCTX-M-1	fw: GTTACAATGTGTGAGAAGCAG	1041	60˚C	[42]
blaCTX-M-1	rev: CCGTTTCCGCTATTACAAAC	1041	60˚C	[42]
tetA(A)	fw: GGTTCACTCGAACGACGTCA	576	50˚C	[43]
tetA(A)	rev: CTGTCCGACAAGTTGCATGA	576	50˚C
tetA(B)	fw: CCTCAGCTTCTCAACGCGTG	633	50˚C
tetA(B)	rev: GCACCTTGCTCATGACTCTT	633	50˚C
sul	fw: CGGCGTGGGCTACCTGAACG		60˚C	[44]
sul	rev: GCCGATCGCGTGAAGTTCCG		60˚C	[44]
cmlA	fw: CCGCCACGGTGTTGTTGTTATC	698	50˚C	[45]
cmlA	rev: CACCTTGCCTGCCCATCATTAG	698	50˚C	[45]
floR	fw: TTTGGWCCGCTMTCRGAC	494	50˚C	[46]
floR	rev: SGAGAARAAGACGAAGAAG	494	50˚C	[46]
mcr-1	fw: AGTCCGTTTGTTCTTGTGGC	320	58 °C	[47]
mcr-1	rev: AGATCCTTGGTCTCGGCTTG	320	58 °C
mcr-5	fw: ATGCGGTTGTCTGCATTTATC	1644	58 °C
mcr-5	rev: TCATTGTGGTTGTCCTTTTCTG	1644	58 °C
chuA	fw: GAC GAA CCA ACG GTC AGG AT	279	55˚C	[27]
chuA	rev: TGC CGC CAG TAC CAA AGA CA	279
yjaA	fw: TGA AGT GTC AGG AGA YGC TG	211
yjaA	rev: ATG RAG AAT GCG TTC CTC AAC	211
tspE4C2	fw: GAG TAA TGT CGG GGC ATT CA	152
tspE4C2	rev: CGC GYC AAC AAA GTA TTR CG	152

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ISSN: 1471-2180

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