Table 3 List of the designed primers and their Tms, IDs, sequences, the amplicon ID and length
Primers ID | Tm oC | primer sequence 5’ > 3’ | Amplicon ID according to database | Amplicon length/ bp | ||
|---|---|---|---|---|---|---|
27 F | 50 | AGAGTTTGATCCTGGCTCAG | 16 S rDNA gene | 1500 | ||
1492R | GGTTACCTTGTTACGACTT | |||||
NarX-F | 55 | GGATGAAATGGCGTCGCTTG | nitrate/nitrite two-component system sensor histidine kinase, NarX | 268 | ||
NarX-R | TTCCCGGCTGTCGTTTTCAT | |||||
NarQ-F | CTGTTCGGCATCGTGATACT | nitrate/nitrite two-component system sensor histidine kinase, NarQ | 266 | |||
NarQ-R | TGCAGCGCCAGATAGTGGTT | |||||
NarK-F | CCGTAGAAGGCGATGAACGA | NarK family nitrate/nitrite MFS transporter | 212 | |||
NarK-R | ACGCAATTTCCGGAGGTCGT | |||||
oxyred-F | CAGATCGTTCAGGCCCATCG | Putative Oxidoreductase, UbiI “SMDB11_RS16455” | 256 | |||
oxyred-R | CGAGCTGATTTCCGAACTGC | |||||
NTR-F | ATGAATTGCTGCACCGGCTC | Putative nitroreductase “SMDB11_RS08680” | 259 | |||
NTR-R | ACGTGATGACGCTCGAAGAG | |||||
NapC/NirT-F | AACCTGCATCGATTGCCACA | NapC/NirT family cytochrome c (nitrate reductase) | 378 | |||
NapC/NirT-R | GTTTCCGCATCGGTCTGAGTC | |||||
AmoA-1 F | GGGGTTTCTACTGGTGGT | alpha subunit of ammonia monooxygenase gene | - | |||
AmoA-1R | CCCCTCKGSAAAGCCTTCTTC [K = G or T; S = G or C] | |||||
NxrA- F | CAGACCGACGTGTGCGAAAG | nitrite oxidase, NxrA | - | |||
NxrA- R | TCCACAAGGAACGGAAGGTC | |||||