Fig. 3

The effect of antibiotic on serum UA and gut microbiota in HUA model mouse. (a) A diagram illustrating the procedure of antibiotic treatment in HUA model mouse. C57BL/6J were randomly assigned to either NC group, hyperuricemia model group (M), or antibiotic-treated hyperuricemia model group (M-Ab). The NC and M groups were fed autoclaved water, while the M-Ab groups were fed the water containing an antibiotic cocktail (ampicillin 250 mg/L neomycin 250 mg/L and metronidazole 50 mg/L were mixed) and were fed the forage containing 5% yeast and 250 kg-1. d-1 potassium oxonate via peritoneal injection. After antibiotic treated for 14 days, all animals were sacrificed using CO2 inhalation. (b) Serum UA levels of groups were shown. (c) The Alpha-diversity among the M-Ab and M groups. (d) The heat map in the M-Ab and M groups. These results are expressed as mean ± SEM.*P < 0.05, **P < 0.01, vs. NC group. HUA, hyperuricemia; M, hyperuricemia model mouse; M-Ab, antibiotic-fed hyperuricemia model mouse; UA, uric acid; NC, control