Table 4 Primer sequence and expected amplicon size of the six selected genes used for qRT-PCR in this study
Gene | Primer Sequence (5’ -3’) | Amplicon size (bp) | Efficiency E (%) | Correlation coefficient (R2) |
|---|---|---|---|---|
groEL | GCACCAGTTCGTCAAATTGC (L) CACTCGTTTGTAGCAGCGTT (R) | 110 | 115 | 1.00 |
ilvC | AAACGGACGCTTTACAAGGC (L) GTCAAAAGAACGACCTGGGC (R) | 131 | 121 | 0.97 |
metI | ACATGGTATTGCATCATTCGCT (L) ATGTGCCGCCGTATAAATCG (R) | 116 | 120 | 0.94 |
metE | CGAAAGCGTGCGTACTTCAA (L) TCTCGGCTTTGTGGGAATGA (R) | 120 | 118 | 0.90 |
icaA | CGACGTTGGCTACTGGGATA (L) TGCTTCCAAAGACCTCCCAA (R) | 150 | 122 | 0.94 |
emp | CGCGTGAATGTAACAACAAACA (L) CTTGTAGTGGGTTTGCGTAGT (R) | 138 | 114 | 0.98 |
rpsT | CTGAAGCACGCAACATTTCAC (L) ACTTTGAGCAGCTTTGTCTACT (R) | 140 | 119 | 0.92 |