Table 2 RNA-seq data validation by RT-qPCR using six selected genes (ilvC, metI, metE, icaA, emp, and rpsT). RNA-seq values represent log₂ fold change of transcripts inferred by bioinformatics prediction. RT-qPCR values represent the mean log₂ fold change in transcripts. The value represents the log2 of the relative fold change between control (S. aureus only) and test (S. aureus with host). For both the analysis, log₂ fold change and adjusted p-value is presented. The p-value of less than 0.05 was significant

Gene Symbol

RNA seq

RT qPCR

RNA-seq

qRT-PCR

ilvc

8.18

35

0.0000

0.0280

metI

2.95

2

0.0000

0.3351

metE

3.8

5

0.0000

0.1472

icaA

2.95

6

0.0009

0.0043

emp

-2.97

-1

0.0004

0.3831

rpst

-2.23

0.7

0.0000

0.0050