Fig. 10

Schematic representation of the in vitro co-culturing of S. aureus with or without tonsillar cells. S. aureus (inoculum) was added to PLL-coated wells containing host media in absence (controls) or presence of monolayer of host cells (tests) at MOI 5 and incubated for 1 or 3 h. Three independent experiments were run in triplicates. The adhered bacteria were collected and plated for either CFU enumeration or RNA extraction. The RNA samples were further processed for RNA-seq followed by DEGs analysis (C1/T1 and C3/T3)