Fig. 7

 L. jensenii 62G and G. vaginalis JCP8151A are metabolically active during the long-term stationary phase. (a) Strains were grown in MSVF at pH 5.0 for 6 and 12 dpi and carbenicillin sufficient to inhibit growth (64 µg/mL for L. jensenii or 1 µg/mL for G. vaginalis) was added. Incubation was continued for an additional 24 h and samples were taken at 7 and 13 dpi and the CFU/mL were determined. (b) Addition of exogenous glycogen to MSVF pH 5.0 at intervals during the long-term stationary phase did not affect growth of L. jensenii or G. vaginalis. A concentrated solution of glycogen in water to a total of 2.5 mg/mL was added to cultures of the strains in MSVF at pH 5.0 at 6 or 12 dpi. Incubation was continued for an additional 24 h and samples were taken at 7 and 13 dpi and the CFU/mL were determined. For both panels: each symbol represents the mean of three independent experiments ± SOM. Two-tailed unpaired t-tests were used to determine significant differences between treatments; ns, no significance; ****, P < 0.0001