Fig. 5From: Glycogen availability and pH variation in a medium simulating vaginal fluid influence the growth of vaginal Lactobacillus species and Gardnerella vaginalisAll strains require glycogen for growth except L. gasseri 63 AM, which uses glucose at pH 5.0. Strains were grown for 15 d in standard MSVF, which contains 10 g/L of glycogen and 10 g/L of glucose, MSVF with half the level of glycogen (MSVF_5Gly) at (a) pH 4.0, (b) pH 4.5, and (c) pH 5.0, and MSVF with no glycogen (MSVF_0Gly) at (d) pH 4.0, (e) pH 4.5, and (f) pH 5.0 Samples were taken at 5-d intervals and the CFU/mL were determined. Each symbol represents the mean of three independent experiments ± SOM. Two-way ANOVA with Tukey’s multiple comparisons posttest was done to determine significant differences between time points across the growth curves. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001Back to article page