Prevalence of Salmonella Typhimurium and Salmonella Enteritidis isolated from poultry meat: virulence and antimicrobial-resistant genes

Table 2 Primer sequences and their annealing temperatures used in this study (Ma et al. 2007)

Resistance Gene	Primer	Nucleotide sequence 50–30	Product size (bp)	Annealing temperature (°C)	Code of antibiotics
tetA	F R	TTGGCATTCTGCATTCACTC GTATAGCTTGCCGGAAGTCG	494	55	TET
tetB	F R	CAGTGCTGTTGTGTCATTAA GCTTGGAATACTGAGTGTAA	571	55	TET
tetC	F R	CTTGAGAGCCTTCAACCCAG ATGGTCGTCATCTACCTGCC	418	55	TET
tetD	F R	GCTCGGTGGTATCTCTGCTC AGCAACAGAATCGGGAACAC	546	55	TET
tetE	F R	TATTAACGGGCTGGCATTTC AGCTGTCAGGTGGGTCAAAC	544	55	TET
tetG	F R	GCTCGGTGGTATCTCTGCTC CAAAGCCCCTTGCTTGTTAC	550	55	TET
sul1	F R	TTTCCTGACCCTGCGCTCTAT GTGCGGACGTAGTCAGCGCCA	793	55	COT
sul2	F R	CCTGTTTCGTCCGACACAGA GAAGCGCAGCCGCAATTCAT	667	55	COT
sul3	F R	ATGAGCAAGATTTTTGGAATCGTAA CTAACCTAGGGCTTTGGTATTT	792	55	COT
cat1	F R	AACCAGACCGTTCAGCTGGAT CCTGCCACTCATCGCAGTAC	549	55	CHL
cat2	F R	AACGGCATGAACCTGAA ATCCCAATGGCATCGTAAAG	547	55	CHL
cat3	F R	ATCGGCATCGGTTACCATGT ATCCCCTTCTTGCTGATATT	310	55	CHL
cmlA	F R	GGCCTCGCTCTTACGTCATC GCGACACCAATACCCACTAGC	662	55	CHL
cmlB	F R	ACTCGGCATGGACATGTACT ACGGACTGCGGAATCCATAG	840	55	CHL
floR	F R	ATGACCACCACACGCCCCG AGACGACTGGCGACTTCTTCG	198	55	CHL
aac(3)11a	F R	CGGCCTGCTGAATCAGTTTC AAAGCCCACGACACCTTCTC	439	55	GEN
aph(3)11a	F R	TCTGAAACATGGCAAAGGTAG AGCCGTTTCTGTAATGAAGGA	582	55	GEN
aac6	F R	TTGGACGCTGAGATATATGA GCTCCTTTTCCAGAATACTT	476	55	GEN
blaTEM-1	F R	CAGCGGTAAGATCCTTGAGA ACTCCCCGTCGTGTAGATAA	643	55	Control
16 S rDNA	F R	AGAGTTTGATCMTGGCTCAG CCGTCAATTCMTTTRAGTTT	907	55	Control

ISSN: 1471-2180