Fig. 5

Mean values of TNFα titer concentrations in two cell growth media. The two media consisted of proliferation (P) and nutrient-reduced differentiation medium (D) containing both uninfected (-) and infected (+) NSC-34 cells, during Day 1–8 after infection with R. helvetica. The concentration of TNFα was calculated using the standard curve. Experiments with P-medium (10% FBS) were performed twice in duplicates, and with D-medium (1.0% FBS) three times in duplicates. No significant rise in titer or significant difference in titer was seen between infected or uninfected cell media (p = 0.2014 (medium P) and p = 0.7619 (medium D). Black filled circles (P+) and triangles (D+) represent infected cell media and unfilled symbols uninfected media (P-/D-).