Table 1 ANOVA, regression analysis, and significance of the Plackett–Burman experimental design to estimate the influential variables for β-glucan biosynthesis by Kluyveromyces lactis
Run Order | Nitrogen conc. (gl−1) | Sulfates conc. (gl−1) | Phosphates conc. (gl−1) | Minerals conc. (ml/l) | Vitamins conc. (gl−1) | β-glucan Conc. (mg/100 ml) | ||
|---|---|---|---|---|---|---|---|---|
1 | 0.9 (-1) | 1.0 (+ 1) | 0.6 (-1) | 15.0 (+ 1) | 0.50 (+ 1) | 285.8 | ||
2 | 9.0 (+ 1) | 0.1 (-1) | 0.6 (-1) | 15.0 (+ 1) | 0.50 (+ 1) | 271.3 | ||
3 | 0.9 (-1) | 0.1 (-1) | 0.6 (-1) | 1.5 (-1) | 0.50 (+ 1) | 210.7 | ||
4 | 9.0 (+ 1) | 1.0 (+ 1) | 0.6 (-1) | 1.5 (-1) | 0.05 (-1) | 181.6 | ||
5 | 0.9 (-1) | 1.0 (+ 1) | 0.6 (-1) | 15.0 (+ 1) | 0.05 (-1) | 218.3 | ||
6 | 9.0 (+ 1) | 1.0 (+ 1) | 0.6 (-1) | 15.0 (+ 1) | 0.50 (+ 1) | 332.5 | ||
7 | 9.0 (+ 1) | 0.1 (-1) | 0.6 (-1) | 1.5 (-1) | 0.05 (-1) | 146.4 | ||
8 | 0.9 (-1) | 0.1 (-1) | 0.6 (-1) | 15.0 (+ 1) | 0.05 (-1) | 083.4 | ||
9 | 0.9 (-1) | 1.0 (+ 1) | 6.0 (+ 1) | 1.5 (-1) | 0.50 (+ 1) | 199.9 | ||
10 | 9.0 (+ 1) | 1.0 (+ 1) | 6.0 (+ 1) | 1.5 (-1) | 0.05 (-1) | 161.5 | ||
11 | 0.9 (-1) | 1.0 (+ 1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.50 (+ 1) | 252.5 | ||
12 | 0.9 (-1) | 0.1 (-1) | 0.6 (-1) | 1.5 (-1) | 0.05 (-1) | 202.3 | ||
13 | 0.9 (-1) | 1.0 (+ 1) | 6.0 (+ 1) | 1.5 (-1) | 0.05 (-1) | 261.3 | ||
14 | 9.0 (+ 1) | 0.1 (-1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.05 (-1) | 241.9 | ||
15 | 9.0 (+ 1) | 1.0 (+ 1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.05 (-1) | 245.1 | ||
16 | 0.9 (-1) | 0.1 (-1) | 6.0 (+ 1) | 1.5 (-1) | 0.50 (+ 1) | 128.9 | ||
17 | 9.0 (+ 1) | 0.1 (-1) | 6.0 (+ 1) | 1.5 (-1) | 0.50 (+ 1) | 278.9 | ||
18 | 9.0 (+ 1) | 1.0 (+ 1) | 0.6 (-1) | 1.5 (-1) | 0.50 (+ 1) | 261.5 | ||
19 | 0.9 (-1) | 0.1 (-1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.05 (-1) | 284.5 | ||
20 | 9.0 (+ 1) | 0.1 (-1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.50 (+ 1) | 281.1 | ||
Model Summary | ||||||||
Source | DF | Adj. SS | Adj. MS | F-Value | P-Value | Effect | Coefficient | T-Value |
Model | 5 | 30540 | 6108 | 1.97 | 0.145 | |||
Linear | 5 | 30540 | 6108 | 1.97 | 0.145 | |||
Constant | 226.5 | 18.21 | ||||||
Nitrogen | 1 | 3754 | 3754 | 1.21 | 0.289 | 27.4 | 13.7 | 1.10 |
Sulfates | 1 | 3667 | 3667 | 1.19 | 0.295 | 27.1 | 13.5 | 1.09 |
Phosphates | 1 | 1012 | 1012 | 0.33 | 0.576 | 14.2 | 7.1 | 0.57 |
Minerals | 1 | 10728 | 10728 | 3.47 | 0.084 | 46.3 | 23.2 | 1.86 |
Vitamins | 1 | 11380 | 11380 | 3.68 | 0.076 | 47.7 | 23.9 | 1.92 |
Error | 14 | 43305 | 3093 | |||||
Total | 19 | 73845 | ||||||