Table 1 Assessing the interaction of DA with candidate proteins by surface plasmon resonance
From: Binding proteins of destruxin A from Metarhizium against insect cell
Function or location | Protein annotation | KD (μM) |
|---|---|---|
Cytoskeleton and cell movement | ||
Actin-4 | 25.6 | |
Laminin subunit gamma-1 | 28.6 | |
Myosin heavy chain, non-muscle | 32.2 | |
Alpha-actinin | 54.9 | |
CAD protein | 38.8 | |
Dynein heavy chain, cytoplasmic | 55.8 | |
Alpha-tubulin | 69.5 | |
Microtubule-actin cross-linking factor 1 | 81.9 | |
Translation | ||
Elongation factor 1 delta | 37.8 | |
EIF3-S8 | 45.0 | |
Elongation factor 1-alpha | 61.1 | |
Eukaryotic translation initiation factor 3 subunit C isoform X1 | 82.8 | |
Translation elongation factor 2 | 85.9 | |
Lysine-tRNA ligase isoform X2 | 99.1 | |
Ubiquitin–proteasome system | ||
E3 UFM1-protein ligase 1 homolog | 45.6 | |
26S protease regulatory subunit 6A-B | 62.8 | |
Ubiquitin carboxyl-terminal hydrolase 5 | 93.4 | |
Phospholipase A-2-activating protein | 95.6 | |
Ubiquitin-like modifier-activating enzyme 1 | 95.8 | |
Polyubiquitin | 99.7 | |
Proteasome 26S non-ATPase subunit 12 isoform X1 | 264 | |
Transcription | ||
ATP-binding cassette sub-family F member 2 isoform X1 | 24.0 | |
Splicing factor proline- and glutamine-rich | 46.6 | |
La-related protein 1 | 54.2 | |
Zinc finger CCCH domain-containing protein 15 homolog | 65.0 | |
Poly A binding protein | 65.6 | |
THO complex subunit 1 | 69.5 | |
mRNA-capping enzyme isoform X1 | 73.5 | |
Symplekin | 79.0 | |
Leucine-rich PPR motif-containing protein | 262 | |
Nucleus | ||
EN protein binding/engrailed nuclear homeoprotein-regulated protein | 52.1 | |
Importin-5 | 35.1 | |
Importin-7 isoform X1 | 58.2 | |
Exportin-1 | 64.6 | |
Importin-7 isoform X2 | 99.6 | |
E3 SUMO-protein ligase RanBP2 | 96.5 | |
Nucleolar and coiled-body phosphoprotein 1 | 67.0 | |
Cell growth-regulating nucleolar protein | 86.1 | |
Nucleolar protein 9 | 99.2 | |
Endoplasmic reticulum | ||
Transitional endoplasmic reticulum ATPase TER94 | 43.0 | |
Dolichyl-diphosphooligosaccharide protein glycotransferase | 73.3 | |
COP complex beta subunit | 60.9 | |
Calnexin | 60.9 | |
Ribosome-binding protein 1 isoform X1 | 46.3 | |
Sarco/endoplasmic reticulum calcium ATPase | 96.8 | |
Peptidase | ||
Prolyl oligopeptidase | 27.0 | |
Protein disulfide isomerase precursor | 44.6 | |
Cytosolic non-specific dipeptidase | 59.3 | |
Xaa-Pro aminopeptidase 1 | 73.7 | |
Presequence protease, mitochondrial | 81.2 | |
Membrane protein | ||
ATP synthase | 40.3 | |
Limbic system-associated membrane protein | 71.0 | |
H + transporting ATP synthase beta subunit isoform 2 | 84.0 | |
Beta-alanine transporter isoform X1 | 97.0 | |
Glycosylated lysosomal membrane protein | 99.8 | |
Others | ||
Uncharacterized protein LOC101744639 | 99.4 | |
Uncharacterized protein LOC101744580 | 97.9 | |
Vacuolar protein sorting-associated protein 13C | 95.2 | |
Muscle glycogen phosphorylase | 91.7 | |
Lysosomal alpha-mannosidase-like_ partial | 89.6 | |
Dicer-2 isoform X1 | 82.0 | |
Failed axon connections isoform X1 | 80.0 | |
Serrate RNA effector molecule homolog isoform X2 | 77.6 | |
U3 small nucleolar RNA-associated protein 14 homolog C | 73.3 | |
Transketolase | 72.9 | |
LETM1 and EF-hand domain-containing protein 1, mitochondrial | 71.6 | |
Cytosolic purine 5'-nucleotidase isoform X2 | 71.2 | |
DnaJ-20 | 69.5 | |
Low-density lipoprotein receptor-related protein 2 | 68.5 | |
Dihydrolipoamide dehydrogenase | 65.9 | |
Ras GTPase-activating protein-binding protein 2 | 62.7 | |
Neutral alpha-glucosidase AB-like precursor | 61.9 | |
Uncharacterized protein LOC101735738 isoform X2 | 58.5 | |
Serine hydroxymethyltransferase | 53.8 | |
Scavenger receptor class B member 3 | 50.1 | |
Uncharacterized protein LOC101735738 isoform X1 | 49.6 | |
Serine/threonine-protein phosphatase 2A | 36.3 | |
Protein scabrous | 469 | |
ATP-citrate synthase-like, partial | 212 | |
ADP-ribosylation factor 2 | 205 | |