New native Bacillus thuringiensis strains induce high insecticidal action against Culex pipiens pallens larvae and adults

Table 1 Lists of primers used for amplification of cry and cyt genes from B. thuringiensis

Genes	Sequence 5’-3’	Product size	TM (°C)	Reference
*Cry1*	CTGGATTTACAGGTGGGGATAT TGAGTCGCTTCGCATATTTGACT	594	52	[44]
*Cry2*	GAGTTTAATCGACAAGTAGATAATTT GGAAAAGAGAATATAAAAATGGCCAG	500	50	[69]
*Cry4*	GCATATGATGTAGCGAAACAAGCC GCGTGACATACCCATTTCCAGGTCC	439	58	[55]
*Cry4Aa*	GGGTATGGCACTCAACCCCACTT GCGTGACATACCCATTTCCAGGTCC	777	50	[79]
*Cry4Ba*	GAGAACACACCTAATCAACCAAT GCGTGACATACCCATTTCCAGGTCC	347	52	[79]
*Cry4A*	TCAAAGATCATTTCAAAATTACATG CGGCTTGATCTATGTCATAATCTGT	459	50	[55]
*Cry4B*	CGTTTTCAAGACCTAATAATATAATACC CGGCTTGATCTATGTCATAATCTGT	321	50	[55]
*Cry10*	TCAATGCTCCATCCAATG CTTGTATAGGCCTTCCTCCG	348	51	[55]
*Cry10Aa*	ATTGTTGGAGTTAGTGCAGG AATACTTTGGATGTGTCTTGAG	995	50	[79]
*Cry11*	CGCTTACAGGATGGATAGG GCTGAAACGGCACGAATATAATA	342	50	[69]
*Cry11Aa*	CCGAACCTACTATTGCGCCA CTCCCTGCTAGGATTCCGTC	470	50	[79]
*Cry11Ba*	TACAGGATGGATAGGGAATGG TAATACTGCCATCTGTTGCTTG	608	52	[79]
*Cry24*	TTATCAATGTTAAGGGATGC ACTGGATCTGTGTATATTTTCCTAG	304	52	[69]
*Cry32*	TGGTCGGGAGAGAATGGATGGA ATGTTTGCGACACCATTTTC	676	48	[69]
*Cry44Aa*	CATTACACGGGGTGCGTTAT CCGCACTTACATGTGTCCAA	444	60	[80]
*Cyt1*	CCTCAATCAACAGCAAGGGTTATT TGCAAACAGGACATTGTATGTGTAATT	477	52	[69]
*Cyt2*	ATTACAAATTGCAAATGGTATTCC TTTCAACATCCACAGTAATTTCAAATGC	356	56	[69]
*Cyt1Aa*	AACTCAAACGAATAACCAAG TGTTCCTTTACTGCTGATAC	300	53	[79]
*Cyt1Ba*	AAGCAAGGGTTATTACATTACG CCAATACTAAGATCAGAGGG	698	54	[79]
*Cyt2Aa*	GCATTAGGAAGACCATTTG AAGGCTAAGAGTTGATATCG	361	53	[79]

ISSN: 1471-2180