Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli

BMC Microbiology

Table 1 Bacterial strains and plasmids used in the study

Strain or Plasmid	Relevant genotype or description	Reference
*C. jejuni* strains
M1cam	Wild-type; derivative of M1	[25, 26]
11168	Wild-type	[27]
81–176	Wild-type	[28]
80512	Wild-type	[29]
80864	Wild-type	[29]
50520408	Wild-type	[29]
*C. coli* strains
15–537360	Wild-type	[30]
H062180535	Wild-type	[31]
H102680185	Wild-type	[31]
CCN182	Wild-type	[31]
*C. hyointestinalis*
ATCC35217	Wild-type	[32]
*C. lari*
ATCC35221	Wild-type	[33]
*E. coli* strains
NEB 5α	F^–φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(r_K^–,m_K⁺) phoA supE44 λ^– thi-1 gyrA96 relA1	New England Biolabs
BL21 pLysS	F⁻ompT hsdS_B(r_B⁻ m_B⁻) gal dcm (DE3) pLysS (Cam^R)	Novagen
Plasmids
pRC1-mariner	mariner Tn (Cm^R) donor plasmid used for in vitro Tn mutagenesis; pRC1 backbone (pSV009 derivative), Amp^R	[23]
pMalC9	Plasmid for expression of Himar1 transposase fused to a maltose binding protein, Amp^R	[34]

ISSN: 1471-2180