Table 2 Comparison of the relative abundance means among the top 20 genera by treatment

Prevotella

Treponema

Bacteroides

Oscillibacter ¹

Clostridium_XlVa

Desulfovibrio

Parabacteroides

Control

29.8750

0.8400

1.5775

1.8825

1.1975

0.4638

2.7010

Cu-MHAC

32.7500

0.7343

0.4158

2.5275

1.4253

0.7847

0.5435

CuSO₄

30.1500

1.4365

0.6033

2.4675

0.6033

0.5585

0.9645

TBCC

28.8250

1.4660

0.5840

1.2625

1.6445

0.6310

0.4895

CV_mean (%)

8.1

65.8

182.0

0.145

44.7

14.3

P -value

Trial

< 0.0001**

0.0086**

0.1741

-

<0.0001**

<0.0001**

0.0982*

Treatment

0.2009

0.1291

0.9817

0.1450

0.0207**

0.0006*

0.2582

P -value for Orthogonal contrasts

Control x All

0.6330

0.3315

0.9809

-

0.2251

0.0013**

0.0589*

Cu-MHAC vs. (CuSO₄ + TBCC)

0.6814

0.5422

0.9995

-

0.0044**

0.0033**

0.6705

CuSO₄ vs. TBCC

0.0460**

0.0373**

0.6903

-

0.5215

0.0042**

0.9644

Treatment

Genus

Clostridium_sensu_

stricto

Succinivibrio

Campylobacter

Fusobacterium ¹

Alloprevotella

Megasphaera

Streptococcus

Control

1.2180

3.2515

1.6853

0.0755

5.0100

1.6950

0.5623

Cu-MHAC

1.1588

2.6115

1.6738

0.0260

3.2875

1.1105

0.7545

CuSO₄

1.2220

5.9400

0.5828

0.7720

3.2275

1.0575

0.9475

TBCC

0.8010

4.0150

1.9038

0.4353

5.6900

0.9105

0.9150

CV_mean (%)

50.2

55.9

142.2

0.615

73.4

32.6

88.4

P -value

Trial

0.4086

0.1974

0.0091**

-

0.0016**

0.2694

0.0001**

Treatment

0.6702

0.2242

0.7304

0.6150

0.1038

0.0686*

0.6839

P -value for Orthogonal contrasts

Control x All

0.6315

0.4784

0.4870

-

0.8135

0.0125**

0.7089

Cu-MHAC vs. (CuSO₄ + TBCC)

0.4892

0.0786*

0.9196

-

0.8283

0.8470

0.2664

CuSO₄ vs. TBCC

0.3796

0.3888

0.3944

-

0.0183**

0.4819

0.9192

Treatment

Genus

Roseburia

Actinobacillus

Helicobacter

Phascolarctobacterium

Acidaminococcus

Escherichia /Shigella

Control

1.9198

0.1473

0.0933

2.0000

1.0195

2.3318

Cu-MHAC

1.4760

0.2163

0.0355

2.8350

0.8313

3.2350

CuSO₄

3.0873

0.5953

0.1985

2.5850

0.5483

3.6100

TBCC

1.8483

0.2998

0.1353

2.8750

0.4458

3.8026

CV_mean (%)

40.6

166.4

97.7

29.8

53.0

60.2

P -value

Trial

<0.000**

0.0235**

0.3746

0.2148

0.0021**

0.0053**

Treatment

0.2500

0.7069

0.1110

0.3879

0.3475

0.1279

P -value for Orthogonal contrasts

Control x All

0.4989

0.3105

0.7716

0.1121

0.2960

0.0245**

Cu-MHAC vs. (CuSO₄ + TBCC)

0.0672*

0.7067

0.0405*

0.5771

0.8475

0.6287

CuSO₄ vs. TBCC

0.7312

0.7139

0.1733

0.9425

0.1476

0.6391